Summary of the article "Borax-Based Gel Electrophoresis: A Novel Approach for RNA Integrity Assessment" published on bioRxiv:

Background & Objective:

The authors propose an alternative agarose gel electrophoresis method using borax buffer, optimized for assessing DNA and RNA integrity. The goal is to develop a simpler, safer, and more cost-effective technique compared to traditional buffers like TBE or TAE.

Developed Technique:

The gel is prepared with borax buffer, offering stable conductivity and high-resolution nucleic acid separation. Various RNA and DNA samples were tested and compared to traditional gels.

Main Results:

Borax gel provides clear RNA band separation, with resolution comparable or superior to classical methods. It is safer, more affordable, and easily implementable in labs.

Conclusion:

This innovative borax gel offers a simple, effective solution for rapid nucleic acid integrity assessment—especially valuable for resource-limited laboratories.

Link to article: bioRxiv – Borax-Based Gel Electrophoresis